Cell preparation is carried out as follows:

the
culture solution is centrifuged; the cell sediment
is placed in a hypo-osmolar KCI solution
(0.075 molar), incubated for about 20 minutes,
and centrifuged again. The resulting cell sediment
is placed in fixative. The fixing solution is
a mixture of methyl alcohol and glacial acetic
acid in a ratio of 3:1. Usually the fixative is
changed two to three times with subsequent
centrifugation. After that, the fixed cells are
taken up in a pipette and dropped onto a slide.
The preparation is stained, and the slide is
covered with a cover glass.