Translocation 4;8

This preparation shows the translocation of
part of the long arm of a chromosome 8 to the
short arm of a chromosome 4 in a patient with
Langer–Giedion syndrome. The hybridization
was done with a 170-kb YAC (yeast artifical
chromosome) that spans the breakpoint of the
translocation in the 8q24 region. Three fluorescent
signals result: over the normal chromosome
8, over the part of 8q24 translocated to
chromosome 4, and over the sequences remaining
on chromosome 8. Chromosomes 4 and 8
were hybridizedwith alphoid probes, which are
specific for the centromere region.